2024

A. Hida, N. Okano, C. Tadokoro, M. Fukunishi, A. Ali Ahmed, K. Takenaka, Y. Tateuchi, K. Fujioka, H. Torii, T. Tajima, J. Kato: Fermented botanical fertilizer controls bacterial wilt of tomatoes caused by Ralstonia pseudosolanacearum, Bioscience, Biotechnology, and Biochemistry, 88(5), 571–576 (2024) doi: 10.1093/bbb/zbae016

T. Tajima, S. Kawaguchi, T. Matsutani, A. Hida, J. Kato: Acclimation of microbial consortia to ammonia and salt in methane fermentation, Fermentation, 10(2), 98 (2024) doi: 10.3390/fermentation10020098

2023

A. Tani, S. Masuda, Y. Fujitani, T. Iga, Y. Haruna, S. Kikuchi, W. Shuaile, H. Lv, S. Katayama, H. Yurimoto, Y. Sakai, J. Kato: Metabolism-linked methylotaxis sensors responsible for plant colonization in Methylobacterium aquaticum strain 22A, Frontiers in Microbiology, 14, 1258452 (2023) doi: 10.3389/fmicb.2023.1258452

G. N. Abdel-Hady, T. Tajima, T. Ikeda, T. Ishida, H. Funabashi, A. Kuroda, R. Hirota: A novel salt- and organic solvent-tolerant phosphite dehydrogenase from Cyanothece sp. ATCC 51142, Front. Bioeng. Biotechnol., 11, 1255582 (2023) doi: 10.3389/fbioe.2023.1255582

L. V. Duc, S. Nagao, M. Mojarrad, Y. Miyagawa, Z.-Y. Li, D. Inoue, T. Tajima, M. Ike: Bioaugmentation with marine sediment-derived microbial consortia in mesophilic anaerobic digestion for enhancing methane production under ammonium or salinity stress, Bioresource Technology, 376, 128853 (2023) doi: 10.1016/j.biortech.2023.128853

2021

G. Luo, S. Fujii, T. Koda, T. Tajima, Y. Sambongi, A. Hida, J. Kato: Unexpectedly high thermostability of an NADP-dependent malic enzyme from a psychrophilic bacterium, Shewanella livingstonensis Ac10, Journal of Bioscience and Bioengineering, 132(5), 445-450 (2021) doi: j.jbiosc.2021.07.005

M. Mojarrad, T. Tajima, A. Hida, J. Kato: Psychrophile-based simple biocatalysts for effective coproduction of 3-hydroxypropionic acid and 1,3-propanediol, Bioscience, Biotechnology, and Biochemistry, 85(3), 728-738 (2021) doi: 10.1093/bbb/zbaa081

M. Tunchai, A. Hida, S. Oku, T. Tajima, J. Kato: Chemotactic disruption as a method to control bacterial wilt caused by Ralstonia pseudosolanacearum, Bioscience, Biotechnology, and Biochemistry, 85(3), 697–702 (2021) doi: 10.1093/bbb/zbaa065

2020

M. Mojarrad, K. Hirai, K. Fuki, T. Tajima, A. Hida, J. Kato: Efficient production of 1,3-propanediol by psychrophile-based simple biocatalysts in Shewanella livingstonensis Ac10 and Shewanella frigidimarina DSM 12253, Journal of Biotechnology, 323, 293-301 (2020) doi: 10.1016/j.jbiotec.2020.09.007

A. Hida, S. Oku, M. Miura, H. Matsuda, T. Tajima, J. Kato: Characterization of methyl-accepting chemotaxis proteins (MCPs) for amino acids in plant-growth-promoting rhizobacterium Pseudomonas protegens CHA0 and enhancement of amino acid chemotaxis by MCP genes overexpression, Bioscience, Biotechnology, and Biochemistry84(9), 1948-1957 (2020) doi:10.1080/09168451.2020.1780112

G. Luo, M. Fujino, S. Nakano, A. Hida, T. Tajima, J. Kato: Accelerating itaconic acid production by increasing membrane permeability of whole-cell biocatalyst based on a psychrophilic bacterium Shewanella livingstonensis Ac10, Journal of Biotechnology312, 56-62 (2020) doi:10.1016/j.jbiotec.2020.03.003

RR. Navarro, Y. Otsuka, K. Matsuo, K. Sasaki, K. Sasaki, T. Hori, H. Habe, M. Nakamura, Y. Nakashimada, K. Kimbara, J. Kato: Combined simultaneous enzymatic saccharification and comminution (SESC) and anaerobic digestion for sustainable biomethane generation from wood lignocellulose and the biochemical characterization of residual sludge solid, Bioresource Technology, 300, 122622 (2020) doi:10.1016/j.biortech.2019.122622

F. Rahayu, T. Tajima, J. Kato, S. Kato, Y. Nakashimada: Ethanol yield and sugar usability in thermophilic ethanol production from lignocellulose hydrolysate by genetically engineered Moorella thermoaceticaJournal of Bioscience and Bioengineering129(2), 160-164 (2020) doi:10.1016/j.jbiosc.2019.08.008

2019

A. Hida, T. Tajima, J. Kato: Two citrate chemoreceptors involved in chemotaxis to citrate and/or citrate-metal complexes in Ralstonia pseudosolanacearumJournal of Bioscience and Bioengineering127(2), 169-175 (2019) doi:10.1016/j.jbiosc.2018.07.014

G. Mahipant, J. Kato, N. Kataoka, AS. Vangnai: An alternative genome-integrated method for undomesticated Bacillus subtilis and related species, J Gen Appl Microbiol65(2), 96-105 (2019) doi:10.2323/jgam.2018.06.001

2018

RR. Navarro, Y. Otsuka, M. Nojiri, S. Ishizuka, M. Nakamura, K. Shikinaka, K. Matsuo, K. Sasaki, K. Sasaki, K. Kimbara, Y. Nakashimada, J. Kato: Simultaneous enzymatic saccharification and comminution for the valorization of lignocellulosic biomass toward natural products, BMC Biotechnol18(1), 79 (2018) doi:10.1186/s12896-018-0487-1

A. Corral-Lugo, M. Matilla, D. Martín-Mora, H. Silva Jiménez, N. M. Torres, J. Kato, A. Hida, S. Oku, M. Conejero-Muriel, J. A. Gavira, T. Krell: High-affinity chemotaxis to histamine mediated by the TlpQ chemoreceptor of the human pathogen Pseudomonas aeruginosaMBio9(6), e01894-18 (2018) doi:10.1128/mBio.01894-18

K. Watanabe, K. H. V. Arafiles, R. Higashi, Y. Okamura, T. Tajima, Y. Matsumura, Y. Nakashimada, K. Matsuyama, T. Aki: Isolation of Aurantiochytrium sp. mutants highly producing carotenoids and improvement of astaxanthin productivity using metabolic information, Journal of Oleo Science67(5), 571-578 (2018) doi:10.5650/jos.ess17230

T. Tajima, K. Tomita, H. Miyahara, K. Watanabe, T. Aki, Y. Okamura, Y. Matsumura, Y. Nakashimada, J. Kato: Efficient conversion of mannitol derived from brown seaweed to fructose for fermentation with a thraustochytrid, Journal of Bioscience and Bioengineering125(2), 180-184 (2018) doi:10.1016/j.jbiosc.2017.09.002

2017

S. Oku, A. Hida, M. Tunchai, T. Tajima, Nakashimada, J. Kato: Involvement of many chemotaxis sensors in negative chemotaxis to ethanol in Ralstonia pseudosolanacearum Ps29, Microbiology163(12), 1880-1889 (2017) doi:10.1099/mic.0.000574

P. Duangkaew, S. Inoue, T. Aki, Y. Nakashimada, Y. Okamura, T. Tajima, Y. Matsumura: Quantitative in situ MS analysis of mannitol decomposition products under hydrothermal conditions, Energy & Fuels31(10), 10866-10873 (2017) doi:10.1021/acs.energyfuels.7b01558

T. Miura, A. Kita, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, J. Kato, Y. Nakashimada: Improved methanization and microbial diversity during batch mode cultivation with repetition of substrate addition using defined organic matter and marine sediment inoculum at seawater salinity, Bioresource Technology245(Pt A), 833-840 (2017) doi:10.1016/j.biortech.2017.09.009

A. Hida, S. Oku, Y. Nakashimada, T. Tajima, J. Kato: Identification of boric acid as a novel chemoattractant and elucidation of its chemoreceptor in Ralstonia pseudosolanacearum Ps29, Scientific Reports7(1), 8609 (2017) doi:10.1038/s41598-017-09176-3

M. Tunchai, A. Hida, S. Oku, Y. Nakashimada, T. Nikata, T. Tajima, J. Kato: Negative chemotaxis of Ralstonia pseudosolanacearum to maleate and identification of the maleate chemosensory protein, Journal of Bioscience and Bioengineering124(6), 647-652 (2017) doi:10.1016/j.jbiosc.2017.07.002

F. Rahayu, Y. Kawai, Y. Iwasaki, K. Yoshida, A. Kita, T. Tajima, J. Kato, K. Murakami, T. Hoshino, Y. Nakashimada:Thermophilic ethanol fermentation from lignocellulose hydrolysate by genetically engineered Moorella thermoacetica,  Bioresource Technology245(Pt B), 1393-1399 (2017) doi:10.1016/j.biortech.2017.05.146

Y. Iwasaki, A. Kita, K. Yoshida, T. Tajima, S. Yano, T. Shou, M. Saito, J. Kato, K. Murakami, Y. Nakashimada: Homolactic acid fermentation by the genetically engineered thermophilic homoacetogen Moorella thermoacetica ATCC39073, Applied and Environmental Microbiology83(8), pii: e00247-17 (2017) doi:10.1128/AEM.00247-17

M. Tunchai, A. Hida, S. Oku, Y. Nakashimada, T. Tajima, J. Kato: Identification and characterization of chemosensors for d-malate, unnatural enantiomer of malate, in Ralstonia pseudosolanacearumMicrobiology163(2), 233-242 (2017) doi:10.1099/mic.0.000408

P. Duangkaew, S. Inoue, T. Aki, Y. Nakashimada, Y. Okamura, T. Tajima, Y. Matsumura: Real-Time Mass Spectrometric Analysis of Hydrothermal Reaction Products, Industrial & Engineering Chemistry Research56(36), 9993-9998 (2017) doi:10.1021/acs.iecr.7b02663

P. Duangkaew, S. Inoue, T. Aki, Y. Nakashimada, Y. Okamura, T. Tajima, Y. Matsumura: In situ mass spectrometry of glucose decomposition under hydrothermal reactions, Korean J. Chem. Eng.34(3), 1-7 (2017) doi:10.1007/s11814-017-0030-4

P. Duangkaew, S. Inoue, T. Aki, Y. Nakashimada, Y. Okamura, T. Tajima, Y. Matsumura: In-situ mass spectroscopic analysis of glucose decomposition under hydrothermal condition: Quantitative analysis for reaction kinetics, Journal of the Japan Petroleum Institute60(2), 101-109 (2017) doi:10.1627/jpi.60.101

R. Mohamad, T. Aki, Y. Nakashimada, Y. Okamura, T.Tajima, Y. Matsumura: Decomposition kinetics of uronic acids obtained from kelp under hydrothermal condition, Journal of the Energy Institute90(2), 185-190 (2017) doi:10.1016/j.joei.2016.02.005

G. Mahipant, A.Paemanee, S. Roytrakul, J. Kato, AS. Vangnai: The significance of proline and glutamate on butanol chaotropic stress in Bacillus subtilis 168, Biotechnol Biofuels10, 122 (2017) doi: 10.1186/s13068-017-0811-3

2016

R. Mohamad, T. Aki, Y. Nakashimada, Y. Okamura, T. Tajima, Y. Matsumura: Kinetics of sorbitol decomposition under hydrothermal condition, Journal of Japan Petroleum Institute59(4), 149-154 (2016) doi:10.1627/jpi.59.149

A. Kita, K. Kobayashi, T. Miura, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, N. Nishio, Y. Nakashimada: High-rate fermentation of acetate to methane under high salinity by acetoclastic methanogens immobilized in marine sediment, Journal of the Japan Petroleum Institute56(1), 9-15 (2016) doi:10.1627/jpi.59.9

A. Kita, T. Miura, S Kawata, T. Yamaguchi, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, J. Kato, N. Nishio, Y. Nakashimada: Bacterial community structure and predicted alginate metabolic pathway in an alginate-degrading bacterial consortium, Journal of Bioscience and Bioengineering121(3), 286-292 (2016) doi:10.1016/j.jbiosc.2015.06.014

A. Kita, K. Suehira, T. Miura, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, N. Nishio, Y. Nakashimada: Characterization of a halotolerant acetoclastic methanogen highly enriched from marine sediment and its application in removal of acetate, Journal of Bioscience and Bioengineering121(2), 196-202 (2016) doi:10.1016/j.jbiosc.2015.05.018

Y. Okamura, S. Nakai, M Ohkawachi, M. Suemitsu, H. Takahashi, T. Aki, Y. Matsumura, T. Tajima, Y. Nakashimada, M. Matsumoto: Isolation and characterization of a lipid-producing bacterium from short-chain fatty acids, Bioresource Technology201, 215-221 (2016) doi:10.1016/j.biortech.2015.11.040

T. Miura, A. Kita, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, J. Kato, Y. Nakashimada:Semi-continuous methane production from undiluted brown algae using a halophilic marine microbial community, Bioresource Technology200, 616-623 (2016) doi:10.1016/j.biortech.2015.10.090

R. Mohamad, T. Aki, Y. Nakashimada, Y. Okamura, T. Tajima, Y. Matsumura: Decomposition kinetics of mannose, its sugar alcohol, and its uronic acid under hydrothermal condition, Journal of Chemical Engineering of Japan49(7), 663-667 (2016) doi:10.1252/jcej.15we164

2015

T. Miura, A. Kita, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, J. Kato, Y. Nakashimada :Effect of salinity on methanogenic propionate degradation by acclimated marine sediment-derived culture, Applied Biochemistry and Biotechnology177(7), 1541-1552 (2015) doi:10.1007/s12010-015-1834-5

R. Matsumoto, T. Aki, Y. Nakashimada, Y. Okamura, T. Tajima, Y. Matsumura: Determination of Mannitol Decomposition Rate under Hydrothermal Pretreatment Condition, Journal of the Japan Petroleum Institute58, 252-255, (2015) doi:10.1627/jpi.58.252

A. Hida, S. Oku, T. Kawasaki, Y. Nakashimada, T. Tajima, J. Kato: Identification of the mcpAand mcpM genes encoding methyl-accepting chemotaxis proteins (MCPs) for amino acids and L-malate and involvement of McpM-mediated chemotaxis in plant infection by Ralstonia pseudosolanacearum (Ralstonia solanacearum)Applied and Environmental Microbiology81(21), 7420-7430 (2015) doi:10.1128/AEM.01870-15

T. Tajima, M. Hamada, Y. Nakashimada, J. Kato: Efficient aspartic acid production by a psychrophile-based simple biocatalyst, Journal of Industrial Microbiology & Biotechnology42(10) 1319-1324, (2015) doi:10.1007/s10295-015-1669-7

A. Kita, T. Miura, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, J. Kato, Y. Nakashimada:Dysgonomonas alginatilytica sp. nov., a novel alginate-degrading bacterium isolated from microbial consortium, International Journal of Systematic and Evolutionary Microbiology65(10), 3570-3575, (2015) doi:10.1099/ijsem.0.000459

N. Kataoka, AS. Vangnai, T. Pongtharangkul, T Tajima, T. Yakushi, K. Matsushita, J. Kato:Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coliJournal of Biotechnology204, 25-32 (2015) doi:10.1016/j.jbiotec.2015.03.027

T. Miura, A. Kita, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, J. Kato, Y. Nakashimada:Improved methane production from brown algae under high salinity by fed-batch acclimation, Bioresource Technology187, 275-281 (2015) doi:10.1016/j.biortech.2015.03.142

Sampedro I, Kato J, Hill JE: Elastin degradation product isodesmosine is a chemoattractant for Pseudomonas aeruginosaMicrobiology161(7), 1496-1503 (2015) doi:10.1099/mic.0.000090

T. Jojima, R. Noburyu, M. Sasaki, T. Tajima, M. Suda, H. Yukawa, M. Inui: Metabolic engineering for improved production of ethanol by Corynebacterium glutamicumApplied Microbiology and Biotechnology 99(3), 1165-1172 (2015) doi:10.1007/s00253-014-6223-4

2014

S. Oku, A. Komatsu, Y. Nakashimada, T. Tajima, J. Kato: Identification of Pseudomonas fluorescens chemotaxis sensory proteins for malate, succinate, and fumarate, and their involvement in root colonization, Microbes and Environments29(4), 413-419 (2014) doi:10.1264/jsme2.ME14128

A. Kongpol, J. Kato, T. Tajima, T. Pongtharangkul, AS. Vangnai: Enhanced 3-methylcatechol production by Pseudomonas putida TODE1 in a two-phase biotransformation system, The Journal of General and Applied Microbiology 60 (4), 183-190 (2014) doi:10.2323/jgam.60.183

KH. Arafiles, H. Iwasaka, Y. Eramoto, Y. Okamura, T. Tajima, Y. Matsumura , Y. Nakashimada, T. Aki: Value-added lipid production from brown seaweed biomass by two-stage fermentation using acetic acid bacterium and thraustochytrid. Applied Microbiology and Biotechnology98 (22), 9207-9216 (2014) doi:10.1007/s00253-014-5980-4

T. Miura, A. Kita, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, J. Kato, Y. Nakashimada:Evaluation of marine sediments as microbial sources for methane production from brown algae under high salinity. Bioresource Technology169, 362-366 (2014) doi:10.1016/j.biortech.2014.07.013

N. Kataoka, AS. Vangnai, H. Ueda, T. Tajima, Y. Nakashimada, J. Kato: Enhancement of (R)-1,3-butanediol production by engineered Escherichia coli using a bioreactor system with strict regulation of overall oxygen transfer coefficient and pH, Bioscience, Biotechnology, and Biochemistry78(4), 695-700 (2014) doi:10.1080/09168451.2014.891933

T. Nitisakulkan, S. Oku, D. Kudo, Y. Nakashimada, T. Tajima, AS. Vangnai, J. Kato:Degradation of chloroanilines by toluene dioxygenase from Pseudomonas putida T57, Journal of Bioscience and Bioengineering117(3), 292-297 (2014) doi:10.1016/j.jbiosc.2013.08.012

R. Matsumoto, T. Aki, Y. Okamura, T. Tajima, Y. Nakashimada, Y. Matsumura: Behavior of organics in kelp during hydrothermal pretreatment: Fundamental characteristics and effect of salt, J. Jpn. Inst. Energy93(5), 531-535 (2014) doi:10.3775/jie.93.531

2013

T. Tajima, K. Fuki, N. Kataoka, D. Kudou, Y. Nakashimada, J. Kato: Construction of a simple biocatalyst using psychrophilic bacterial cells and its application for efficient 3-hydroxypropionaldehyde production from glycerol, AMB Express3(1), 69 (2013) doi:10.1186/2191-0855-3-69

AS. Vangnai, K. Takeuchi, S. Oku, N. Kataoka, T. Nitisakulkan, T. Tajima, J. Kato:Identification of CtpL as a chromosomally-encoded chemoreceptor for 4-chloroaniline and catechol in Pseudomonas aeruginosa PAO1, Applied and Environmental Microbiology79(23), 7241-7248 (2013) doi:10.1128/AEM.02428-13

Y. Iwasaki, A. Kita, S. Sakai, K. Takaoka, S. Yano, T. Tajima, J. Kato, N. Nishio, K. Murakami, Y. Nakashimada: Engineering of a functional thermostable kanamycin resistance marker for use in Moorella thermoacetica ATCC39073, FEMS Microbiology Letters343(1), 8-12 (2013) doi:10.1111/1574-6968.12113

N. Kataoka, AS. Vangnai, T. Tajima, Y. Nakashimada, J. Kato: Improvement of (R)-1,3-butanediol production by engineered Escherichia coliJournal of Bioscience and Bioengineering115(5), 475-480 (2013) doi:10.1016/j.jbiosc.2012.11.025

A. Kita, Y. Iwasaki, S. Sakai, S. Okuto, K. Takaoka, T. Suzuki, S. Yano, S. Sawayama, T. Tajima, J. Kato, N. Nishio, K. Murakami, Y. Nakashimada: Development of genetic transformation and heterologous expression system in carboxydotrophic thermophilic acetogen Moorella thermoaceticaJournal of Bioscience and Bioengineering115(4), 347-352 (2013) doi:10.1016/j.jbiosc.2012.10.013

2012

S. Oku, A. Komatsu, T. Tajima, Y. Nakashimada, J. Kato: Identification of chemotaxis sensory proteins for amino acids in Pseudomonas fluorescens Pf0-1 and their involvement in chemotaxis to tomato root exudate and root colonization, Microbes and Environments27(4), 462-469 (2012) doi:10.1264/jsme2.ME12005

T. Tajima, N. Hayashida, R. Matsumura, A. Omura, Y. Nakashimada, J. Kato: Isolation and characterization of tetrahydrofuran-degrading Rhodococcus aetherivorans strain M8, Process Biochemistry47(11), 1665-1669 (2012) doi:10.1016/j.procbio.2011.08.009

AS. Vangnai, N. Kataoka, S. Soonglerdsongpha, C. Kalambaheti, T. Tajima, J. Kato: Construction and application of an Escherichia coli bioreporter for aniline and chloroaniline detection, Journal of Industrial Microbiology & Biotechnology39(12), 1801-1810 (2012) doi:10.1007/s10295-012-1180-3

K. Honda, M. Imura, K. Okano, T. Omasa, J. Kato, H. Ohtake: Identification of the replication region of a 111-kb circular plasmid from Rhodococcus opacus B-4 by λ red recombination-based deletion analysis, Bioscience, Biotechnology, and Biochemistry76(9), 1758-1764 (2012) doi:10.1271/bbb.120362

N. Wangrangsimagul, K. Klinsakul, A. S. Vangnai, J. Wongkongkatep, P. Inprakhon, K. Honda, H. Ohtake, J. Kato, T. Pongtharangkul: Bioproduction of vanillin using an organic solvent-tolerant Brevibacillus agri 13, Applied Microbiology and Biotechnology93(2), 555-563 (2012) doi:10.1007/s00253-011-3510-1

A. Siriphongphaew, P. Pisnupong, J. Wongkongkatep, P. Inprakhon, A. S. Vangnai, K. Honda, H. Ohtake, J. Kato, J. Ogawa, S. Shimizu, V. B. Urlacher, R. D. Schmid, T. Pongtharangkul: Development of a whole-cell biocatalyst co-expressing P450 monooxygenase and glucose dehydrogenase for synthesis of epoxyhexane, Applied Microbiology and Biotechnology95(2), 357-367 (2012) doi:10.1007/s00253-012-4039-7

A. Kongpol, J. Kato, T. Tajima, AS. Vangnai: Characterization of acetonitrile-tolerant marine bacterium Exiguobacterium sp SBH81 and its tolerance mechanism, Microbes and Environments27(1), 30-35 (2012) doi:10.1264/jsme2.ME11228


2011

N. Kataoka, T. Tajima, J. Kato, W. Rachadech, AS. Vangnai: Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host, AMB Express1(1), 10 (2011). doi:10.1186/2191-0855-1-10

S. Sakai, Y. Takaki, S. Shimamura, M. Sekine, T. Tajima, H. Kosugi, N. Ichikawa, E. Tasumi, A. T. Hiraki, A. Shimizu, Y. Kato, R. Nishiko, K. Mori, N. Fujita, H. Imachi, K. Takai: Genome sequence of a mesophilic hydrogenotrophic methanogen Methanocella paludicola, the first cultivated representative of the order MethanocellalesProS One6(7), e22898 (2011) 10.1371/journal.pone.0022898

S. Oku, H. Kinoshita, T. Tajima, J. Kato: Identification and characterization of methyl-accepting chemotaxis proteins for trichloroethylene in Pseudomonas putida F1. Journal of Environmental Biotechnology11(1-2), 83-87 (2011)