Most whole cell biocatalysts have some problems with yields and productivities because of various metabolites produced as byproducts and limitations of substrate uptake. We propose a psychrophile-based simple biocatalyst (PSCat) for efficient bio-production using mesophilic enzymes expressed in psychrophilic Shewanella livingstonensis Ac10 cells whose basic metabolism was inactivated by heat treatment. The PSCat was applied for 3-HPA and aspartic acid production by using Klebsiella pneumoniae dhaB genes. 3-HPA and aspartic acid was stoichiometrically produced with the complete consumption of glycerol at a high production rate.
G. Luo, M. Fujino, S. Nakano, A. Hida, T. Tajima, J. Kato: Accelerating itaconic acid production by increasing membrane permeability of whole-cell biocatalyst based on a psychrophilic bacterium Shewanella livingstonensis Ac10, Journal of Biotechnology, in press, doi:10.1016/j.jbiotec.2020.03.003
T. Tajima, K. Tomita, H. Miyahara, K. Watanabe, T. Aki, Y. Okamura, Y. Matsumura, Y. Nakashimada, J. Kato: Efficient conversion of mannitol derived from brown seaweed to fructose for fermentation with a thraustochytrid, Journal of Bioscience and Bioengineering, 125(2), 180-184 (2018) doi:10.1016/j.jbiosc.2017.09.002
T. Tajima, M. Hamada, Y. Nakashimada, J. Kato: Efficient aspartic acid production by a psychrophile-based simple biocatalyst, Journal of Industrial Microbiology & Biotechnology, 42(10) 1319-1324, (2015) doi:10.1007/s10295-015-1669-7
T. Tajima, K. Fuki, N. Kataoka, D. Kudou, Y. Nakashimada, J. Kato: Construction of a simple biocatalyst using psychrophilic bacterial cells and its application for efficient 3-hydroxypropionaldehyde production from glycerol, AMB Express, 3(1), 69 (2013) doi: 10.1186/2191-0855-3-69